dna-methylation-profiling-gene-specific-profiling-skov3-h19

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RNA isolation / purification Bacteria - Gram positive Lactobacillus amylovorus Experiment

The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads can be a best alternative.

RNA RNA isolation / purification Bacteria Gram positive Lactobacillus amylovorus

RNA isolation / purification Bacteria - Gram positive Listeria monocytogens Experiment

RNA RNA isolation / purification Bacteria Gram positive Listeria monocytogens

RNA isolation / purification Bacteria - Gram positive Staphylococcus epidermidis Experiment

RNA RNA isolation / purification Bacteria Gram positive Staphylococcus epidermidis

RNA isolation / purification Bacteria - Gram positive Staphylococcus saprophycitius Experiment

RNA RNA isolation / purification Bacteria Gram positive Staphylococcus saprophycitius

RNA isolation / purification Bacteria - Gram positive Streptococcus pyogenes Experiment

RNA RNA isolation / purification Bacteria Gram positive Streptococcus pyogenes

RNA sequencing Human - H9 Experiment

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

RNA RNA sequencing Human H9

PCR Multiplex PCR - Viral Experiment

DNA PCR Multiplex PCR Viral

PCR Conventional / Qualitative PCR - Experiment

DNA PCR Conventional / Qualitative PCR

TruSeq RNA Library Prep Kit v2 Product

Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Human - H1819

Products Illumina TruSeq RNA Library Prep Kit v2

PCR Quantitative real-time PCR - Experiment

DNA PCR Quantitative real-time PCR

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