siRNA / miRNA gene silencing Human Melanoma cells (501 Mel and SK Mel 28)

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using EpiTect Bisulfite Kit to perform DNA methylation profiling Gene specific profiling - SKOV3 AGR2

Get tips on using Monoclonal Mouse Anti-Human Actin (Smooth Muscle) (Concentrate) Clone 1A4 to perform Immunohistochemistry Mouse - SMA

Get tips on using Human/Mouse GFR alpha-2/GDNF R alpha-2 Antibody to perform Immunohistochemistry Mouse - Gfrα2

Get tips on using PureLink™ HiPure Plasmid Filter Purification Kits - for Midi and Maxi preparation of Plasmid DNA to perform Plasmid Isolation Citrobacter koseri

Get tips on using CelLytic™ NuCLEAR™ Extraction Kit to perform Protein isolation Mammalian cells - Human eutopic endometrial stromal cells

Get tips on using EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion to perform Cell Isolation Monocyte

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

Get tips on using EasySep™ Direct Human Naïve B Cell Isolation Kit to perform Cell Isolation Naive B cell

Get tips on using MACSprep™ HLA B/T Cell Isolation Kit, human to perform Cell Isolation HLA B/T