siRNA / miRNA gene silencing Human Primary Endometrial Stromal Cells

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Get tips on using Gibco™ DMEM, high glucose to perform Stem cell culture media Human salivary gland stem cells

Products Fisher Scientific Gibco™ DMEM, high glucose

Get tips on using Gibco™DMEM/F-12 to perform Stem cell culture media Human salivary gland stem cells

Products Thermo Fisher Scientific Gibco™DMEM/F-12

Get tips on using Gibco™DMEM/F-12 to perform Stem cell culture media Human intestinal stem cells/organoids

Products Thermo Fisher Scientific Gibco™DMEM/F-12

Get tips on using LC3B Antibody Kit for Autophagy to perform Autophagy assay cell type - Human neural progenitor cells (NPC)

Products Thermo Fisher Scientific LC3B Antibody Kit for Autophagy

Get tips on using DeadEnd™ Fluorometric TUNEL System to perform TUNEL assay cell type - HeLa cells human cervical cancer

Products Promega DeadEnd™ Fluorometric TUNEL System

Get tips on using KnockOut™ Serum Replacement - Multi-Species to perform Stem cell Differentiation media Human Limbal Epithelial cells

Products Thermo Fisher Scientific KnockOut™ Serum Replacement - Multi-Species

Get tips on using Anti-LC3 antibody produced in rabbit to perform Autophagy assay cell type - Human osteosarcoma cancer cells

Products Sigma-Aldrich Anti-LC3 antibody produced in rabbit

Get tips on using PE Annexin V Apoptosis Detection Kit I to perform Apoptosis assay cell type - Human T-cells

Products BD Biosciences PE Annexin V Apoptosis Detection Kit I

Get tips on using FITC Annexin V Apoptosis Detection Kit I to perform Apoptosis assay cell type - Human T-cells

Products BD Biosciences FITC Annexin V Apoptosis Detection Kit I

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Cellular assays Apoptosis assay cell type T-cells Mouse (CD4+ and CD8+)

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