Site Directed Mutagenesis (SDM) Monkey Point mutation Cos-7

Get tips on using Bsp1286I NEB#R0120 to perform Restriction Enzymes Bsp1286I / SduI

Get tips on using Human Syndecan-1 DuoSet ELISA to perform ELISA Human - SDC1

Get tips on using Human Syndecan-1 ELISA Kit (CD138) (ab46506) to perform ELISA Human - SDC1

Get tips on using Anti-COX IV antibody - Mitochondrial Loading Control (ab16056) to perform Western blotting COX4

Get tips on using JetPrime to perform DNA transfection Mammalian cells - Immortalized cell lines COS7

Get tips on using pRSFDuet™-1 DNA - Novagen to perform CRISPR Hamster - Deletion CHO-K1 COSMC

Get tips on using ICAfectin®442 siRNA transfection to perform DNA transfection Mammalian cells - Immortalized cell lines COS7

Get tips on using Oris™ Cell Migration Assay - Collagen I Coated to perform Cell migration / Invasion cell type - HaCat

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Get tips on using Color-coded Prestained Protein Marker, Low Range (1.7-42 kDa) #13070 to perform Protein Ladder Prestained