siRNA / miRNA gene silencing Human

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Get tips on using PowerPlex® 18D System to perform Cell line authentication Human iPSC cells derived from peripheral blood mononuclear cells

Get tips on using Neural Progenitor Medium 2 to perform Stem cell Differentiation media Differentiation of Human PSC into Neural progenitor cells

Get tips on using Lipofectamine® LTX Reagent to perform DNA transfection Mammalian cells - Primary cells Human aortic smooth muscle cells (HOSMC)

Get tips on using Gibco™DMEM/F-12 to perform Stem cell culture media Human Fetal brain-derived neural stem cells

Get tips on using Gibco™ MEM α, Nucleosides to perform Stem cell Differentiation media Human oogonial stem cells differentiation into oocytes

Get tips on using STEMdiff™ Pancreatic Progenitor Kit to perform Stem cell Differentiation media Differentiation of Human hESCs into pancreatic progenitors

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Cells - primary human coronary artery smooth muscle cells

Get tips on using DeadEnd™ Fluorometric TUNEL System to perform TUNEL assay cell type - A549, NCI-H460, H1299 human alveolar carcinoma

Get tips on using ROS-Glo™ H2O2 Assay to perform ROS assay cell type - BEAS-2B human bronchial epithelial cell line