Get tips on using Gentra Puregene Blood Kit to perform DNA isolation / purification Cells - Primary cells Bone marrow mononuclear cells
Get tips on using Gentra Puregene Blood Kit to perform DNA isolation / purification Cells - Immortalized cell lines Lymphoblastoid cell lines
Get tips on using QIAamp DNA Blood Mini Kit to perform DNA isolation / purification Cells - Immortalized cell lines HEK 293T
Get tips on using DNeasy Blood and Tissue Kit (250) to perform DNA isolation / purification Cells - Immortalized cell lines Loucy
Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Bacteria - Gram positive piezophilic bacteria [AT7 and AT12 Strains]
Get tips on using FitAmp Blood and Cultured Cell DNA Extraction Kit to perform DNA isolation / purification Cells - Immortalized cell lines SH-SY5Y
Get tips on using Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) to perform Flow cytometry Anti-bodies Mouse - CD16/CD32
Though DNA quantification is but one small step in the multifaceted DNA sample preparation workflow, it can have large implications on the performance and validity of conclusions drawn from downstream assays. Major challenges include accuracy, precision, reproducibility, and detection of present contamination. Among UV spectrophotometry, fluorescence and real-time PCR based methods, the quantification method should be chosen based on the requirement of the downstream assay.
Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,
Get tips on using BBL™ Brain Heart Infusion Broth, BD to perform Bacterial cell culture media Clostridum botulinum
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