shRNA gene silencing Mouse Prostate cancer cell lines (DU145 and PC3)

Get tips on using Smooth Muscle Cell Medium to perform Mammalian cell culture media HAOSMC

Get tips on using Canine Endothelial Cell Media to perform Mammalian cell culture media CnAOEC

Get tips on using Bovine Endothelial Cell Media to perform Mammalian cell culture media BAOEC

Get tips on using Cell Death Detection ELISAPLUS to perform Apoptosis assay cell type - THP-1

Get tips on using Cell Death Detection ELISAPLUS to perform Apoptosis assay cell type - CaOV-3

Get tips on using Cultrex® Cell Migration Assay to perform Cell migration / Invasion cell type - Huh7

Get tips on using Cultrex® Cell Migration Assay to perform Cell migration / Invasion cell type - HepG2

Get tips on using ORIS™ Cell Migration Assay to perform Cell migration / Invasion cell type - FaDu

Get tips on using ORIS™ Cell Migration Assay to perform Cell migration / Invasion cell type - HeLa

The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.