Get tips on using Porcine Endothelial Cell Growth Medium to perform Mammalian cell culture media PCAEC
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Get tips on using Corning® Hepatocyte Culture Media Kit, 500 mL to perform 3D Cell Culture Media Human bladder cancer organoid
The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.
DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.
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Get tips on using Cell Culture Contamination Detection Kit to perform Cell Culture Contamination Detection Kit Fungi
Get tips on using Cell Culture Contamination Detection Kit to perform Cell Culture Contamination Detection Kit Bacteria
Get tips on using Cell Lysis Buffer (10X) to perform Protein isolation Mammalian cells - HepG2
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