Stem cell culture media

Get tips on using EasySep™ Human Cord Blood CD34 Positive Selection Kit II to perform Cell Isolation CD34+ cells

The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.

Get tips on using Nucleofector™ Kits for Human T Cells to perform DNA transfection Mammalian cells - Immortalized cell lines iPSC

Get tips on using DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit to perform ROS assay cell type - human primary corneal epithelial cells

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines Chang Liver cells

Get tips on using DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit to perform ROS assay cell type - H9c2 rat cardiomyocytes

Get tips on using CellROX™ Deep Red Reagent, for oxidative stress detection to perform ROS assay cell type - mouse splenocytes

Get tips on using Fluoro H202 to perform ROS assay cell type - Capan-2, PANC-1 pancreatic carcinoma

Get tips on using CometAssay Electrophoresis System II to perform DNA Damage Assay Human Skin Fibroblast Cell (FSK)

Get tips on using LC3B monoclonal antibody (2G6) to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)