rna-isolation-purification-bacteria-gram-negative-helicobacter-pylori

Get tips on using QuantiTect Virus Kit to perform RNA quantification qPCR

Get tips on using Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade to perform ChIP H3K36Me3 - Sheep Rat -NA-

Get tips on using Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade to perform ChIP H3K27me3 - Sheep Rat YFP Tag

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Rat - Gingival tissue

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Rat - Liver tissue

RNAi or RNA interference is a common method to suppress gene expression in vitro/in vivo by utilizing the inherent microRNA machinery, without introducing a total gene knockout. miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid-mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time-consuming, but provide a more permanent expression of RNAi in the cells and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines.

Get tips on using RNeasy PowerClean Pro Cleanup Kit (50) to perform Removal of contamination in RNA Melanin contamination

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Mouse - C2C12

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Human - H9

Get tips on using Cell Counting Kit-8 to perform RNA quantification Coloremetric