ChIP H3K27me3 Rabbit Donkey

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate to perform Flowcytometry Secondary Antibody - Goat Rabbit Alexa Fluor 488

Get tips on using Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (ab9050) to perform ChIP Anti-bodies H3K36me3

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Rat - Heart

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Rat - H4IIE

Get tips on using Magna ChIP™ A - Chromatin Immunoprecipitation Kit to perform ChIP Rat - Liver

Get tips on using Magna ChIP™ A - Chromatin Immunoprecipitation Kit to perform ChIP Rat - Brain

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Mouse - Brain

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Human - SW480

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Human - ASM