DNA methylation profiling Gene specific profiling Caco-1

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Get tips on using HyClone Dulbecco's Modified Eagle Medium (DMEM)/ F12 1:1: Liquid to perform 3D Cell Culture Media hiPSC-derived cortical organoids

Products Cytiva HyClone Dulbecco's Modified Eagle Medium (DMEM)/ F12 1:1: Liquid

Get tips on using HyClone Dulbecco's Modified Eagle Medium (DMEM)/ F12 1:1: Liquid to perform 3D Cell Culture Media hiPSC-derived forebrain organoids

Products Cytiva HyClone Dulbecco's Modified Eagle Medium (DMEM)/ F12 1:1: Liquid

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Human - Caco-2

Products Active Motif ChIP-IT® Express Chromatin Immunoprecipitation Kits

Get tips on using Apoptosis/ Necrosis Assay Kit (blue, green, red) (ab176749) to perform Necrosis Caco-2

Products Abcam Apoptosis/ Necrosis Assay Kit (blue, green, red) (ab176749)

Get tips on using Apoptosis/ Necrosis Assay Kit (blue, green, red) (ab176749) to perform Ne Caco-2

Products Abcam Apoptosis/ Necrosis Assay Kit (blue, green, red) (ab176749)

Get tips on using jetPEI® DNA transfection, HTS application to perform DNA transfection Mammalian cells - Immortalized cell lines PANC-1

Products Polyplus transfections jetPEI® DNA transfection, HTS application

Get tips on using HyClone Dulbecco's Modified Eagle Medium (DMEM)/ F12 1:1: Liquid to perform Stem cell Differentiation media hDPSCs differentiation into adipogenic cells

Products Cytiva HyClone Dulbecco's Modified Eagle Medium (DMEM)/ F12 1:1: Liquid

Get tips on using HyClone Dulbecco's Modified Eagle Medium (DMEM)/ F12 1:1: Liquid to perform Stem cell Differentiation media hDPSCs differentiation into osteogenic cells

Products Cytiva HyClone Dulbecco's Modified Eagle Medium (DMEM)/ F12 1:1: Liquid

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay luciferase negative control (luciferase vector)

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay luciferase HepG2 and Huh7 cells

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