DNA methylation profiling Gene specific profiling Rat whole pituitary glands

Get tips on using ON-TARGETplus Rat Arhgef6 (363509) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - MTLn3 α-Pix/Arhgef6

Get tips on using ON-TARGETplus Rat Arhgef7 (114559) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - MTLn3 β-Pix/Arhgef7

Get tips on using ON-TARGETplus Rat Itgb1 (24511) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - MTLn3 β1 integrin/Itgb1

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Mouse Adrenal glands

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Human Mammary glands

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Human Adrenal glands

Get tips on using Orai1 Rat siRNA Oligo Duplex (Locus ID 304496) to perform siRNA / miRNA gene silencing Rat - RBL-2H3 Orai1

Get tips on using Rat GE 4x44K v3 Microarray Kit to perform Microarray Gene expression arrays - Rat pancreas tissue Cyanine 3 & cyanine 5