DNA transfection Mammalian cells

- Found 7511 results

I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?

Discussions siRNA/RNAi/miRNA transfection human

Get tips on using EZ DNA Methylation-Direct Kit to perform PCR Methylation specific PCR - Mammalian DNA

Products Zymo Research EZ DNA Methylation-Direct Kit

Get tips on using EZ DNA Methylation-Gold Kit to perform PCR Methylation specific PCR - Mammalian DNA

Products Zymo Research EZ DNA Methylation-Gold Kit

Get tips on using Hot Start Taq DNA Polymerase to perform PCR Hot start PCR - Mammalian DNA

Products GenScript Hot Start Taq DNA Polymerase

Get tips on using Platinum™ Taq DNA Polymerase to perform PCR Hot start PCR - Mammalian DNA

Products Thermo Fisher Scientific Platinum™ Taq DNA Polymerase

Get tips on using FastStart™ Taq DNA Polymerase to perform PCR Conventional / Qualitative PCR - mammalian DNA

Products Sigma-Aldrich FastStart™ Taq DNA Polymerase

Get tips on using Platinum™ Taq DNA Polymerase to perform PCR Conventional / Qualitative PCR - mammalian DNA

Products Thermo Fisher Scientific Platinum™ Taq DNA Polymerase

DNA DNA isolation / purification Cells Immortalized cell lines H1 hESc

Get tips on using M-PER™ Mammalian Protein Extraction Reagent to perform Protein isolation Mammalian cells - Human aortic endothelial cells

Products Thermo Fisher Scientific M-PER™ Mammalian Protein Extraction Reagent

Get tips on using GenomONE™-Neo HVJ-E Membrane Fusion Transfection Kit to perform siRNA / miRNA gene silencing Human - U937 MK2 (MAPK Kinase 2) Viral vectors

Products Cosmo Bio GenomONE™-Neo HVJ-E Membrane Fusion Transfection Kit

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms