shRNA gene silencing Human Islets of langerhans Negative control (scrambled)

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using Gentra Puregene Cell Kit to perform DNA isolation / purification Cells - Primary cells Human primary keratinocytes

Get tips on using PyroMark CpG Assays to perform DNA methylation profiling Whole genome profiling - human whole blood

Get tips on using CYTAG® CGH Labeling kit to perform Microarray Comperative genomic hybridization - Human Colon adenocarcinoma

Get tips on using miRCURY Exosome Cell/Urine/CSF Kit to perform Purification of extracellular vesicles Exosomes - Seminal plasma

Get tips on using RNeasy PowerClean Pro Cleanup Kit (50) to perform Removal of contamination in RNA Melanin contamination

Get tips on using Strep-Tactin Superflow Plus (10 ml) to perform Protein tag Purification of Strep-tagged proteins

Get tips on using Ni-NTA Fast Start Kit (6) to perform Protein tag Purification of His-tagged proteins

Get tips on using Penta·His Antibody, BSA-free (100 ug) to perform Protein tag Detection of His-tagged proteins

Get tips on using Tetra·His Antibody, BSA-free (100 µg) to perform Protein tag Detection of His-tagged proteins