Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary bovine pulmonary artery endothelial cells
Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - primary porcine airway epithelial cells
Get tips on using QIAGEN Proteinase K (10 ml) to perform RNA isolation / purification Cells - primary mouse morula cells
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Human Thyroid gland
Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Human Spinal cord
Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Human Seminal vesicles
Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Human Mammary glands
Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Human Fallopian tubes
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