RNA isolation / purification Tissue Human

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Get tips on using Ovation® RNA Amplification System V2 to perform Microarray RNA amplification & Labeling - Mouse cochlaea Biotin

Products NuGEN Ovation® RNA Amplification System V2

Get tips on using CD163 Antibody, anti-human, PE-Vio® 770, REAfinity™ to perform Flow cytometry Anti-bodies Human - CD163

Products Miltenyibiotec CD163 Antibody, anti-human, PE-Vio® 770, REAfinity™

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Cellular assays Live / Dead assay mammalian cells MDA-MB-231 human breast cancer cells

Get tips on using SiRNA silencing human Eph receptor B4, Id: s243 to perform siRNA / miRNA gene silencing Human - HNSCC cell line Eph receptor B4 Polymer / Lipid

Products Thermo Fisher Scientific SiRNA silencing human Eph receptor B4, Id: s243

Get tips on using SiRNA silencing human Eph receptor B4, Id: 533 to perform siRNA / miRNA gene silencing Human - HNSCC cell line Eph receptor B4 Polymer / Lipid

Products Thermo Fisher Scientific SiRNA silencing human Eph receptor B4, Id: 533

Get tips on using Purified Mouse Anti-Human MLH1 Clone G168-728 (RUO) to perform Immunohistochemistry Human - MLH1

Products BD Biosciences Purified Mouse Anti-Human MLH1 Clone G168-728 (RUO)

Get tips on using Monoclonal Mouse Anti-Human Androgen Receptor (Concentrate) Clone AR441 to perform Immunohistochemistry Human - AR

Products Agilent Technologies Monoclonal Mouse Anti-Human Androgen Receptor (Concentrate) Clone AR441

Get tips on using Monoclonal Mouse Anti-Human Cytokeratin 20 (Concentrate) Clone Ks20.8 to perform Immunohistochemistry Human - CK20

Products Agilent Technologies Monoclonal Mouse Anti-Human Cytokeratin 20 (Concentrate) Clone Ks20.8

Get tips on using SurePrint G3 Human Gene Expression 8x60K v2 Microarray Kit to perform Microarray Human - PCOS

Products Agilent Technologies SurePrint G3 Human Gene Expression 8x60K v2 Microarray Kit

Get tips on using Human Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit to perform ELISA Human - ShhN

Products R&D Systems Human Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit

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