CRISPR Rat Deletion INS-1 832/13

DNA ladder is typically used as a reference to estimate the size of unknown DNA samples that are separated based on their mobility in an electrical field. The critical points for running a DNA ladder are compatibility with running buffer, agarose gel percentage, and choosing the correct range of DNA ladder for sizing DNA molecules.

I intend to use iScript cDNA Synthesis Kit in order to synthesize cDNA for qPCR. I have confirmed that my RNA is pure however, according to my extraction protocol I have suspended the RNA in TE buffer containing 1mM EDTA. Will the presence of EDTA have an effect on cDNA synthesis?

Get tips on using Integrin β1 Antibody (102DF5): sc-73610 to perform Western blotting ITGB1

Get tips on using IMAGEN™ Influenza Virus A and B Kit using Direct Immunofluorescence Assay to perform Cell Culture Contamination Detection Kit Virus

Wound healing assay can be challenging due to inconsistencies and variations while making a wound on the confluent cell monolayer, consequently leads to wounds of varying sizes and widths. Moreover, this assay causes damage to the cells that are at the edge of the wound, which can prevent cell migration into the wound site and healing. The best solution is to use the standard wound healing assay kits using either combs or inserts to make a defined wound field or gap and prevent the well-to-well variation in these assays.

Get tips on using Q-Plex™ Mouse Cytokine – Inflammation (14-plex) to perform ELISA Mouse - GM-CSF

Get tips on using RAC1 Antibody to perform Western blotting Rac1

Get tips on using ON-TARGETplus Mouse Fdps (110196) siRNA - Individual, to perform siRNA / miRNA gene silencing Mouse - MC3T3-E1 Fdps

Get tips on using RAC1 Polyclonal Antibody to perform Western blotting Rac1

Get tips on using ON-TARGETplus Human AHR (196) siRNA - Individual to perform siRNA / miRNA gene silencing Human - MDA-MB-231 AHR