CRISPR Human Activation

Get tips on using Human LBP ELISA Kit (ab213805) to perform ELISA Human - LBP

Get tips on using Human HSP70/HSPA1A DuoSet ELISA to perform ELISA Human - HSP70

Get tips on using Human Fibronectin ELISA Kit (ab108847) to perform ELISA Human - Fibronectin

Get tips on using I-FABP, Human, ELISA kit to perform ELISA Human - FABP2

Get tips on using Human BDNF ELISA Kit (ab99978) to perform ELISA Human - BDNF

Get tips on using Human/Mouse BDNF DuoSet ELISA to perform ELISA Human - BDNF

Get tips on using Human Adiponectin ELISA Kit (ab108786) to perform ELISA Human - Adiponectin

Get tips on using Human Adiponectin/Acrp30 DuoSet ELISA to perform ELISA Human - Adiponectin

Wound healing assay can be challenging due to inconsistencies and variations while making a wound on the confluent cell monolayer, consequently leads to wounds of varying sizes and widths. Moreover, this assay causes damage to the cells that are at the edge of the wound, which can prevent cell migration into the wound site and healing. The best solution is to use the standard wound healing assay kits using either combs or inserts to make a defined wound field or gap and prevent the well-to-well variation in these assays.

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.