DNA transfection Mammalian cells

Get tips on using RNA-Bee to perform RNA isolation / purification Cells - immortalized A2780ADR

Get tips on using RNA-Bee to perform RNA isolation / purification Cells - immortalized A2780

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized A2780

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized A253

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized CHO

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized MDCK

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized CMT12

Get tips on using gRNA_Cloning Vector to perform CRISPR Mouse - Deletion RMA cells Trh4

Get tips on using DeadEnd™ Colorimetric TUNEL System to perform TUNEL assay cell type - Mouse skeletal muscle cells

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.