Immunohistochemistry Anti-Glial Fibrillary Acidic Protein (GFAP) Mouse Human

Get tips on using pTip-QC2-gi_21221796 to perform Protein Expression Prokaryotic cells - R. erythropolis putative tetR-family transcriptional regulatory protein

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.

Get tips on using ExpiCHO™ Expression System Kit to perform Protein expression and purification Mammalian cells - CHO-K1 SUMO protein

Get tips on using PARP-1 C-terminal antibody (pAb) to perform ChIP Anti-bodies PARP

Get tips on using Sall4 Antibody (EE-30): sc-101147 to perform ChIP Anti-bodies Sall4

Get tips on using ChIPAb+™ HDAC1 Antibody, rabbit polyclonal to perform ChIP Anti-bodies HDAC1

Get tips on using Acetyl-Histone H3 (Lys27) Antibody #4353 to perform ChIP Anti-bodies H3K27ac

Get tips on using TFIIB Antibody (D-3): sc-271736 to perform ChIP Anti-bodies TFIIB