Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.
Get tips on using pSVA1470 to perform Protein Expression Prokaryotic cells - S. acidocaldarius MalR
Get tips on using pMSP3535H3 to perform Protein Expression Prokaryotic cells - L. lactis Cry5B
Get tips on using pTRK1061 to perform Protein Expression Prokaryotic cells - L. lactis Cry5B
Get tips on using pQEhCP1 to perform Protein Expression Prokaryotic cells - E. coli EhCP1
Get tips on using pET22β to perform Protein Expression Prokaryotic cells - E. coli CPB
Get tips on using pSVA937 to perform Protein Expression Prokaryotic cells - E. coli MalR
Get tips on using pJS102 to perform Protein Expression Prokaryotic cells - E. coli GFPmut2
Get tips on using pT7lacO2 to perform Protein Expression Prokaryotic cells - E. coli Lycopene
Get tips on using pKD46 to perform Protein Expression Prokaryotic cells - E. coli GFPuv
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment