PCR Quantitative real-time PCR

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Bacteria - Gram negative Bordetella pertussis

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Bacteria - Gram negative Bordetella pertussis

Get tips on using pSpCas9(BB)-2A-Puro (PX459) to perform CRISPR Human - Repression GLT25D1

Get tips on using pHR-SFFV-dCas9-BFP-KRAB to perform CRISPR Human - Repression CXCR4

Get tips on using pSpCas9(BB)-2A-Puro (PX459) to perform CRISPR Human - Deletion NOX4

Generally isolating RNA from Gram-negative bacteria is easy, however keeping your working environment clean and RNase free (use RNase inhibitor) is essential. Some common points to keep in mind: a) Use fresh samples for isolation or store them by freezing in RNA stabilizing buffer until use. b) Choose the bacterial input amounts carefully, to ensure buffer volumes are adequate and not to overload the columns.

Generally isolating RNA from Gram-negative bacteria is easy, however keeping your working environment clean and RNase free (use RNase inhibitor) is essential. Some common points to keep in mind: a) Use fresh samples for isolation or store them by freezing in RNA stabilizing buffer until use. b) Choose the bacterial input amounts carefully, to ensure buffer volumes are adequate and not to overload the columns.

Get tips on using EndoFree Plasmid Mega Kit (5) to perform DNA isolation / purification Plasmid purification

Get tips on using High Pure Plasmid Isolation Kit to perform Plasmid Isolation E.coli Oneshot Top10

Get tips on using Porcine Endothelial Cell Growth Medium to perform Mammalian cell culture media PAOEC