shRNA gene silencing Human Islets of langerhans SOX2

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Get tips on using Global DNA Methylation ELISA to perform DNA methylation profiling Whole genome profiling - HCT116, HTC15 human colon cancer cells

Get tips on using CpGenome Universal DNA Modification Kit to perform DNA methylation profiling Whole genome profiling - OVCAR-3 human ovarian cancer

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I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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