Protein Expression Prokaryotic cells M. smegmatis

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Mouse primary lung epithelial cells-organoids

Get tips on using StemSpan™ SFEM to perform Stem cell Differentiation media hiPSCs differentiation into mesodermal lineage cells

Get tips on using Gibco™DMEM/F-12 to perform Stem cell culture media Mouse trophoblast stem cells

Get tips on using PureLink Genomic DNA Mini Kit to perform DNA isolation / purification Cells - Immortalized cell lines 3T3

Get tips on using PureLink Genomic DNA Mini Kit to perform DNA isolation / purification Cells - Immortalized cell lines HeLa

Get tips on using DeadEnd™ Colorimetric TUNEL System to perform TUNEL assay cell type - Mouse skeletal muscle cells

Get tips on using LC3A (D50G8) XP® Rabbit mAb to perform Autophagy assay cell type - Vestibular schwannoma cells

Get tips on using LC3B (D11) XP® Rabbit mAb to perform Autophagy assay cell type - Vestibular schwannoma cells

Get tips on using LC3A/B (D3U4C) XP® Rabbit mAb to perform Autophagy assay cell type - K562 cells