siRNA / miRNA gene silencing Rat Retinal stem cells

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using Atg5 (D5F5U) Rabbit mAb to perform Autophagy assay cell type - K562 cells

Get tips on using Cytoselect™ Cell Viability and Cytotoxicity Assay to perform Live / Dead assay mammalian cells - MDA-MB-231 human breast cancer cells

Get tips on using LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit, for UV excitation to perform Live / Dead assay mammalian cells - Spleen cells

Get tips on using GenJet™ In Vitro DNA Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines COS7

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Mouse Muscle

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Mouse Liver

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Mouse Kidney

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Mouse Brain

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Mouse Bone