Get tips on using Mouse Shh-N ELISA Kit to perform ELISA Mouse - ShhN
Get tips on using Anti-LC3B antibody produced in rabbit to perform Western blot LC3B - Rabbit Human -NA-
Get tips on using LC3 Antibody (APG8B) (N-term) to perform Autophagy assay cell type - KYSE450
Get tips on using LC3 Antibody (APG8B) (N-term) to perform Autophagy assay cell type - OE21
Get tips on using LC3 Antibody (APG8B) (N-term) to perform Autophagy assay cell type - A549
Get tips on using LC3 Antibody (APG8B) (N-term) to perform Autophagy assay cell type - HepG2
Get tips on using β-catenin Antibody (E-5): sc-7963 to perform Western blotting eta-catenin
Get tips on using β Tubulin Antibody (TUB 2.1): sc-58886 to perform Western blotting Tubulin beta
Get tips on using Purified Mouse Anti-Nucleoporin p62 to perform Western blot p62/SQSTM1 - Mouse IgG2b Human -NA-
Protein ladders are a set of standards known as molecular weight proteins that are utilized to identify the approximate size of a protein molecule run on a PAGE gel electrophoresis. The challenges in running the ladders are the choice of appropriate protein standard as it is used as visual evidence of protein migration, transfer efficiency, and positive control. Suitable protein markers can be selected on the basis of required properties and applications, i.e., fluorescent ladder, IEF, 2D SDS-PAGE ladder, natural ladder with an isoelectric point, and optimized ladders for Western Blot chemiluminescence detection. The key factors for running a distinct protein ladder are buffer conditions, charge/voltage at migration time, and the gel's concentration.
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