Get tips on using EpiTect PCR Control DNA Set (100) to perform PCR ORNi-PCR - Plasmid DNA
Get tips on using Ion AmpliSeq™ Library Kit 2.0 to perform PCR Multiplex PCR - Mammalian DNA
Get tips on using PCR SuperMix to perform PCR Conventional / Qualitative PCR - mammalian DNA
A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. The resulting amplicons are generally detected by gel electrophoresis and for some further applications like cloning, sequencing, amplicon product needs to be recovered from the gel and subsequently purified. However, non-specific product amplification and primer-dimer formation during set-up make gel extraction difficult. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.
A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. The resulting amplicons are generally detected by gel electrophoresis and for some further applications like cloning, sequencing, amplicon product needs to be recovered from the gel and subsequently purified. However, non-specific product amplification and primer-dimer formation during set-up make gel extraction difficult. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.
Get tips on using AmpliTaq Gold™ 360 DNA Polymerase to perform PCR Multiplex PCR - Bacterial DNA
Get tips on using Taq PCR Master Mix Kit to perform PCR Conventional / Qualitative PCR - mammalian DNA
Get tips on using Illustra GFX PCR DNA and Gel Band Purification kit to perform DNA gel extraction / PCR product purification Product size < 15Kb
Get tips on using QuantiNova Probe PCR Kit (2500) to perform PCR Quantitative real-time PCR - Mammalian DNA
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