pcr-multiplex-pcr-mammalian-dna

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality hot-start DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. A quantitative, real-time PCR reaction typically includes all of that plus a probe that can be detected fluorescently as the reaction runs, with no gel required. for detection. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. A quantitative, real-time PCR reaction typically includes all of that plus a probe that can be detected fluorescently as the reaction runs, with no gel required. for detection. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

Get tips on using TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG to perform PCR Multiplex PCR - Mammalian DNA

Get tips on using PerfeCTa MultiPlex qPCR SuperMix to perform PCR Multiplex PCR - Bacterial DNA

Get tips on using Promega PCR Master Mix to perform PCR Multiplex PCR - Bacterial DNA

Get tips on using AmpliTaq Gold™ DNA Polymerase with Buffer II and MgCl2 to perform PCR Multiplex PCR - Mammalian DNA