DNA isolation / purification Tissue

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Get tips on using AllPrep DNA/RNA Micro Kit to perform RNA isolation / purification Tissue - Human Blood / Serum / Plasma / Buffy coat

Get tips on using Chromous Genomic DNA isolation kit to perform DNA isolation / purification Bacteria - Gram positive Bacillus subtilis

Get tips on using PAXgene Tissue miRNA Kit to perform RNA isolation / purification Tissue - rat brain tissue

Get tips on using PAXgene Tissue miRNA Kit to perform RNA isolation / purification Tissue - rat spleen tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Get tips on using PAXgene Tissue miRNA Kit to perform RNA isolation / purification Tissue - rat heart muscle tissue

Get tips on using MasterPure™ Complete DNA and RNA Purification Kit to perform DNA isolation / purification Cells - Immortalized cell lines C2C12

Get tips on using RNeasy Lipid Tissue Mini Kit to perform RNA isolation / purification Tissue - mouse kidney tissue

Get tips on using RNeasy Lipid Tissue Mini Kit to perform RNA isolation / purification Tissue - human adipose tissue