Site Directed Mutagenesis (SDM) Human Point mutation Caco-2

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using CD61-FITC, SZ21, 2 mL, ASR to perform Flow cytometry Anti-bodies Human - CD61

Get tips on using CD22-PE, SJ10.1H11, 2 mL, ASR to perform Flow cytometry Anti-bodies Human - CD22

Get tips on using CD30-PE, HRS4, 2 mL, ASR to perform Flow cytometry Anti-bodies Human - CD30

Get tips on using CD41-PE, P2, 2 mL, ASR to perform Flow cytometry Anti-bodies Human - CD41

Get tips on using Cox-2 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - B16-F10 COX-2

Get tips on using pPIC9K-duIL-2 to perform Protein Expression Eukaryotic cells - P. pastoris rduIL-2

Get tips on using Rock-2 siRNA and shRNA Plasmids (h) to perform siRNA / miRNA gene silencing Human - HT-1376 ROCK2

Get tips on using CD31-FITC, 5.6E, 2 mL, ASR to perform Flow cytometry Anti-bodies Human - CD31/PECAM-1

Get tips on using TACS® 2 TdT Fluorescein Kit to perform TUNEL assay cell type - HeLa cells human cervical cancer