siRNA / miRNA gene silencing Human MSTO-211H

Get tips on using Anti-mTOR antibody to perform Autophagy assay cell type - HepG2

Get tips on using mTOR Antibody #2972 to perform Autophagy assay cell type - SH-SY5Y

Get tips on using Anti-mTOR antibody [53E11] to perform Autophagy assay cell type - HepG2

Get tips on using Phospho-mTOR (Ser2448) Antibody to perform Autophagy assay cell type - HEK 293

Get tips on using Phospho-mTOR (Ser2448) Antibody to perform Autophagy assay cell type - SH-SY5Y

Get tips on using mTOR (7C10) Rabbit mAb to perform Autophagy assay cell type - SH-SY5Y

Get tips on using mTOR Inhibitor XI, Torin1 - Calbiochem to perform Autophagy assay cell type - GL261

Get tips on using mTOR Inhibitor XI, Torin1 - Calbiochem to perform Autophagy assay cell type - GL15

Get tips on using mTOR Inhibitor XI, Torin1 - Calbiochem to perform Autophagy assay cell type - U87MG

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.