siRNA / miRNA gene silencing Human SKOV-3

Get tips on using Phusion Site-Directed Mutagenesis Kit to perform Site Directed Mutagenesis (SDM) Human - Point mutation HEK293 MDR3

Get tips on using MEBSTAIN Apoptosis TUNEL Kit Direct to perform TUNEL assay cell type - PANC-1 human pancriatic cancer

Get tips on using DeadEnd™ Colorimetric TUNEL System to perform TUNEL assay cell type - FaDu human squamous cell carcinoma

Get tips on using DeadEnd™ Fluorometric TUNEL System to perform TUNEL assay cell type - SK-MEL-2 human melanoma

Get tips on using AllPrep DNA/RNA Mini Kit to perform RNA isolation / purification Cells - primary human endometrial stromal cells

Get tips on using DeadEnd™ Fluorometric TUNEL System to perform TUNEL assay cell type - HeLa cells human cervical cancer

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using H2A.X Phosphorylation Assay Kit (Flow Cytometry) to perform DNA Damage Assay Human Skin Fibroblast Cell (FSK)

Get tips on using RNeasy Protect Cell Mini Kit (50) to perform RNA isolation / purification Cells - primary human mononuclear cells

Get tips on using CD31-FITC, 5.6E, 2 mL, ASR to perform Flow cytometry Anti-bodies Human - CD31/PECAM-1