siRNA / miRNA gene silencing Rat NRK

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

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Get tips on using TurboCapture 96 mRNA Kit (5) to perform mRNA / Ribonucleoprotein isolation / purification mRNA

Get tips on using TurboCapture 384 mRNA Kit (5) to perform mRNA / Ribonucleoprotein isolation / purification mRNA

Get tips on using Oligotex mRNA Midi Kit (12) to perform mRNA / Ribonucleoprotein isolation / purification polyA+ mRNA

Get tips on using Oligotex mRNA Mini Kit (12) to perform mRNA / Ribonucleoprotein isolation / purification polyA+ mRNA

Get tips on using MagAttract Direct mRNA M48 Kit (192) to perform mRNA / Ribonucleoprotein isolation / purification mRNA

Get tips on using PCR Sequencing Kit to perform Whole Genome Amplification Human

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.