siRNA / miRNA gene silencing Human LN-18

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized LN15

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized LNCaP

Get tips on using STEMdiff™ Trilineage Differentiation Kit to perform Stem cell Differentiation media Differentiation of Human primed induced pluripotent stem cells (UMN PCBC16iPS) into naive pluripotent stem cells

Get tips on using OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence) to perform ROS assay cell type - PLHC-1, SK-HEP-1, Hep3b, HepG2 human hepatocellular carcinoma

Get tips on using APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU to perform TUNEL assay cell type - A549, NCI-H460, H1299 human alveolar carcinoma

Get tips on using RPMI 1640, 1X to perform Mammalian cell culture media LNCaP

Get tips on using Annexin-V-FLUOS Staining Kit to perform Necrosis LNCaP

Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized LNCaP

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized LNCaP