DNA isolation / purification Bacteria Gram positive

Get tips on using UltraClean® Mini Plasmid Prep Kits to perform Plasmid Isolation Pediococcus acidilactici

Get tips on using PureLink™ Quick Plasmid Miniprep Kit to perform Plasmid Isolation Aeromonas spp.

Get tips on using HiYield Plus™ Plasmid Mini Kit to perform Plasmid Isolation Campylobacter concisus

Get tips on using E.Z.N.A.® Plasmid Mini Kit II to perform Plasmid Isolation Shigella sonnei

Get tips on using E.Z.N.A.®Yeast Plasmid Mini Kit to perform Plasmid Isolation S. cerevisiae

Get tips on using PureLink™ HiPure Plasmid Maxiprep Kit to perform Plasmid Isolation DH10Bac (Bacmid)

Get tips on using MagniSort™ Human pan-Monocyte Enrichment Kit to perform Cell Isolation Monocyte

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.