Western blot 1,4 β-N-acetyl-D-glucosamine Triticum vulgaris Bacteria

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Get tips on using DeadEnd™ Fluorometric TUNEL System to perform TUNEL assay cell type - A549, NCI-H460, H1299 human alveolar carcinoma

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Get tips on using Alexa Fluor® 488 Annexin V/Dead Cell Apoptosis Kit to perform Necrosis HeLa

Products Thermo Fisher Scientific Alexa Fluor® 488 Annexin V/Dead Cell Apoptosis Kit

Get tips on using Alexa Fluor® 488 Annexin V/Dead Cell Apoptosis Kit to perform Necrosis A549

Products Thermo Fisher Scientific Alexa Fluor® 488 Annexin V/Dead Cell Apoptosis Kit

Get tips on using Gibco™Essential 8™ Medium to perform Stem cell Differentiation media hiPSC differentiation into Human Neuronal cells

Products Thermo Fisher Scientific Gibco™Essential 8™ Medium

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay luciferase negative control (luciferase vector)

Get tips on using Flot1 Rat siRNA Oligo Duplex (Locus ID 64665) to perform siRNA / miRNA gene silencing Rat - NRCM Flot1

Products OriGene Flot1 Rat siRNA Oligo Duplex (Locus ID 64665)

Get tips on using Flot2 Rat siRNA Oligo Duplex (Locus ID 83764) to perform siRNA / miRNA gene silencing Rat - NRCM Flot2

Products OriGene Flot2 Rat siRNA Oligo Duplex (Locus ID 83764)

Get tips on using ApoAlert™ DNA Fragmentation Assay Kit to perform TUNEL assay cell type - A549, NCI-H460, H1299 human alveolar carcinoma

Products Takara Bio Inc ApoAlert™ DNA Fragmentation Assay Kit

DNA isolation and purification is the first critical step in sample preparation that helps ensure optimal performance of downstream assays like PCR, microarrays, and sequencing. Failure in yielding high-quality DNA would be the major reason that DNA doesn't work for the downstream application. To circumvent this, one should follow the recommended storage conditions to minimize DNA degradation by nucleases and shouldn't overload the purification system.

DNA DNA isolation / purification Cells Primary cells Mouse embryonic fibroblast (MEF)

Get tips on using Gibco™ DMEM/F-12, GlutaMAX™ supplement to perform Stem cell culture media Neocortical induction from SFEBq

Products Thermo Fisher Scientific Gibco™ DMEM/F-12, GlutaMAX™ supplement

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