siRNA / miRNA gene silencing Human HT-29

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - immortalized HEK 293

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized HEK 293

Get tips on using BECN1 Antibody (H-300) to perform Autophagy assay cell type - HEK 293

Get tips on using Phospho-mTOR (Ser2448) Antibody to perform Autophagy assay cell type - HEK 293

Get tips on using GABARAP (E1J4E) Rabbit mAb to perform Autophagy assay cell type - HEK 293

Get tips on using Atg16L1 (D6D5) Rabbit mAb to perform Autophagy assay cell type - HEK 293

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using One-step TUNEL Assay Kit to perform DNA Damage Assay HEK 293T

Get tips on using DMEM–Dulbecco's Modified Eagle Medium to perform Mammalian cell culture media 293T

Get tips on using Dulbecco’s Modified Eagle’s Medium (DMEM) to perform Mammalian cell culture media 293T