siRNA / RNAi /miRNA transfection Mouse Glomerular Mesangial cells

Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized PC-3

Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized A-172

Get tips on using RNAprep pure Kit to perform RNA isolation / purification Cells - immortalized BRL 3A

Get tips on using illustra RNAspin Mini Kit to perform RNA isolation / purification Cells - immortalized BRL 3A

Get tips on using STEMdiff™ Trilineage Differentiation Kit to perform Stem cell Differentiation media Differentiation of Human primed induced pluripotent stem cells (UMN PCBC16iPS) into naive pluripotent stem cells

Get tips on using ISOGEN to perform RNA isolation / purification Cells - immortalized CHO

Get tips on using RNA-Bee to perform RNA isolation / purification Cells - immortalized 59M

Get tips on using RNA-Bee to perform RNA isolation / purification Cells - immortalized A2780ADR

Get tips on using RNA-Bee to perform RNA isolation / purification Cells - immortalized A2780

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.