Analysis of DNA fragments

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. The resulting amplicons are generally detected by gel electrophoresis and for some further applications like cloning, sequencing, amplicon product needs to be recovered from the gel and subsequently purified. However, non-specific product amplification and primer-dimer formation during set-up make gel extraction difficult. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

Get tips on using DNA Ligation Kit to perform DNA ligation

Get tips on using T4 DNA Ligase to perform DNA ligation

Get tips on using Fast DNA Ladder to perform DNA Ladder Fast

Get tips on using Supercoiled DNA Ladder to perform DNA Ladder Supercoiled

Get tips on using Supermix DNA Ladder to perform DNA Ladder 500 bp

Get tips on using 100bp DNA Ladder to perform DNA Ladder 100 bp

Get tips on using 1kb DNA Ladder to perform DNA Ladder 1 kb

Get tips on using DreamTaq DNA Polymerases to perform PCR Conventional / Qualitative PCR - bacterial DNA

Get tips on using Biotools DNA Polymerase to perform PCR Conventional / Qualitative PCR - bacterial DNA