A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
Get tips on using ATP5A1 Monoclonal Antibody (7H10BD4F9) to perform Western blotting ATP5A
Get tips on using IMAGEN™ Respiratory Virus Screen Kit using Direct Immunofluorescence Assay to perform Cell Culture Contamination Detection Kit Virus
Get tips on using IMAGEN™ Parainfluenza Virus Group Kit using Direct Immunofluorescence Assay to perform Cell Culture Contamination Detection Kit Virus
Get tips on using Bax Antibody (4H32): sc-70407 to perform Western blotting Bax
Get tips on using mTOR (7C10) Rabbit mAb #2983 to perform Western blotting mTOR
Get tips on using Anti-Fas Antibody, clone 7C10 to perform Western blotting Fas
Get tips on using IMAGEN™ Respiratory Syncytial Virus Kit (RSV) using Direct Immunofluorescence Assay to perform Cell Culture Contamination Detection Kit Virus
Get tips on using mTOR (7C10) Rabbit mAb to perform Autophagy assay cell type - SH-SY5Y
Get tips on using VEGF Antibody (C-1): sc-7269 to perform Immunohistochemistry Mouse - VEGFA
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment