The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay HT1080
Get tips on using Dulbecco’s Modified Eagle’s Medium - high glucose to perform Stem cell culture media hTrophoblasts
Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Mouse - HT22
Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - HT29
Get tips on using LC3B (D11) XP® Rabbit mAb to perform Autophagy assay cell type - HT1080
Get tips on using LC3B (D11) XP® Rabbit mAb to perform Autophagy assay cell type - HT1080
Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - HT1080
Get tips on using Thiazolyl Blue Tetrazolium Bromide to perform Cell cytotoxicity / Proliferation assay cell type - HT22 mouse hippocampal cells
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment