siRNA / miRNA gene silencing Human T47-D

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GeneRead Size Selection Kit (50) Product

Get tips on using GeneRead Size Selection Kit (50) to perform Whole Genome Amplification NGS library purification

Products Qiagen GeneRead Size Selection Kit (50)

Plasmid Isolation Proteus mirabilis Experiment

Plasmid isolation is an important technique in molecular biology or any kind of genetic editing. It involves amplifying plasmids overnight by transforming them into competent bacterial cells. The desired colonies of these bacteria can then be grown in shaker cultures, at appropriate shaking speed, oxygen availability and temperature. These liquid cultures can then be ultracentrifuged to pellet the bacteria, which are then used for plasmid isolation. The bacteria are first resuspended in a buffer, then lysed, neutralized, purified in a column, eluted, precipitated with ethanol and then resuspended. During plasmid isolation, it is important to lyse cells quickly because lysing bacteria for too long may lead to irreversible denaturing of the plasmid. Usually, alkaline lysis is used for isolation because it is a mild treatment. It isolates plasmid DNA and other cell components such as proteins by breaking cells apart with an alkaline solution. Precipitation removes the proteins, and the plasmid DNA recovers with alcohol precipitation. Resuspension and lysis buffers should be mixed thoroughly in order to prevent the DNA from breaking into smaller fragments. This is because broken gDNA can reanneal and remain in the solution, without binding to the column.

DNA Plasmid Isolation Proteus mirabilis

Impact of using FBS Discussion

I have tried to fabricate Liver organoids and would like to study the impact of FBS on healthy and tumor organoids. Since the compositions of FBS is unknown, do you recommend any alternatives like Human platelet lysate, etc?

Discussions Impact of using FBS

GeneRead DNA FFPE Kit Product

Get tips on using GeneRead DNA FFPE Kit to perform DNA isolation / purification Tissue - FFPE samples

Products Qiagen GeneRead DNA FFPE Kit

GeneArt™ Site-Directed Mutagenesis System Product

Get tips on using GeneArt™ Site-Directed Mutagenesis System to perform Site Directed Mutagenesis (SDM) Dog - Point mutation MDCK SCRIB