RNA sequencing

Get tips on using Rat NGF/NGF Beta PicoKine™ ELISA Kit to perform ELISA Rat - beta-NGF

Get tips on using Rat Lipocalin-2/NGAL PicoKine™ ELISA Kit to perform ELISA Rat - NGAL/LCN2

Get tips on using Rat KIM-1 TIM-1 ELISA Kit (ab119597) to perform ELISA Rat - KIM-1

Get tips on using Rat heme oxygenase 1,HO-1 ELISA Kit to perform ELISA Rat - HO-1

Get tips on using Rat soluble E-selectin,sE-selectin ELISA Kit to perform ELISA Rat - sE-Selectin

Get tips on using siGENOME Human RAB5A (5868) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - BT-20 Rab5a

Get tips on using LC3A/B (D3U4C) XP® Rabbit mAb to perform Autophagy assay cell type - RAW 264.7

Though DNA quantification is but one small step in the multifaceted DNA sample preparation workflow, it can have large implications on the performance and validity of conclusions drawn from downstream assays. Major challenges include accuracy, precision, reproducibility, and detection of present contamination. Among UV spectrophotometry, fluorescence and real-time PCR based methods, the quantification method should be chosen based on the requirement of the downstream assay.

Though DNA quantification is but one small step in the multifaceted DNA sample preparation workflow, it can have large implications on the performance and validity of conclusions drawn from downstream assays. Major challenges include accuracy, precision, reproducibility, and detection of present contamination. Among UV spectrophotometry, fluorescence and real-time PCR based methods, the quantification method should be chosen based on the requirement of the downstream assay.

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.