shRNA gene silencing Mouse RGC-5

Get tips on using SimpleChIP® Plus Sonication Chromatin IP Kit #56383 to perform ChIP Human - AGS

Get tips on using MycoAlert® Mycoplasma Detection Kit (50 Tests) to perform Cell Culture Contamination Detection Kit Mycoplasma

Get tips on using MycoAlert® Mycoplasma Detection Kit (50 Tests) to perform Cell Culture Contamination Detection Kit Mycoplasma

Get tips on using mericon DNA Bacteria Plus Kit (50) to perform DNA isolation / purification Bacteria - Gram positive Clostridium botulinum

Get tips on using QIAamp Fast DNA Stool Mini Kit (50) to perform DNA isolation / purification Tissue - fecal sample

Get tips on using QIAamp DSP Viral RNA Mini Kit (50) to perform RNA isolation / purification Viral - Dengue virus

Get tips on using RNeasy Protect Cell Mini Kit (50) to perform RNA isolation / purification Cells - primary human mononuclear cells

Get tips on using Corning® 500 mL RPMI 1640 to perform 3D Cell Culture Media hiPSC-derived cardiac organoids

Get tips on using CD31-FITC, 5.6E, 2 mL, ASR to perform Flow cytometry Anti-bodies Human - CD31/PECAM-1

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.