DNA isolation / purification Cells

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU to perform DNA Damage Assay U-2 OS

Get tips on using QIAGEN Large-Construct Kit to perform Plasmid Isolation Shiga toxin-producing E. coli

Get tips on using NucleoBond® Xtra Midi / Maxi to perform Plasmid Isolation Medicago truncatula BAC clone

Get tips on using Qproteome Bacterial Protein Prep Kit to perform Protein isolation Bacteria - Salmonella paratyphi A

Get tips on using Trichloroacetic Acid Solution, MP Biomedicals™ to perform Protein isolation Bacteria - Borrelia burgdorferi

Get tips on using PureYield™ Plasmid Miniprep System Technical Bulletin to perform Plasmid Isolation Klebsiella pneumoniae

Get tips on using PureLink™ HiPure Plasmid Filter Midiprep Kit to perform Plasmid Isolation Enterobacter aerogenes

Get tips on using PureYield™ Plasmid Miniprep System Technical Bulletin to perform Plasmid Isolation Agrobacterium tumefaciens

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.