reporter-gene-assay-luciferase-bhk-21-baby-hamster-kidney-cells

Get tips on using LC3A/B (D3U4C) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #13082 to perform Autophagy assay cell type - RAW 264.7

I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?

Get tips on using BLOCK-iT™ Adenoviral RNAi Expression System, pAd/BLOCK-iT™-DEST RNAi Gateway Vector to perform shRNA gene silencing Mouse - P19 Foxm1

Get tips on using ProcartaPlex Human Cytokine Panel 1B, 25 plex to perform ELISA (kit) Human Serum Cytokine measurements (Multiplex assay) - -NA- Human -NA-

Get tips on using Dead Cell Apoptosis Kit with Annexin V Alexa Fluor™ 488 & Propidium Iodide (PI) to perform Apoptosis assay cell type - RPMI-8226

Get tips on using Dead Cell Apoptosis Kit with Annexin V Alexa Fluor™ 488 & Propidium Iodide (PI) to perform Apoptosis assay cell type - SH-SY5Y

Get tips on using PL_MmBak1 to perform Protein Expression Eukaryotic cells - CHO Bak1

Get tips on using pET-21b-VP24 to perform Protein Expression Prokaryotic cells - E. coli VP24

Get tips on using pET-21a-IFNT to perform Protein Expression Prokaryotic cells - E. coli IFNT

Get tips on using JetPrime to perform DNA transfection Mammalian cells - Primary cells Human chondrocytes