RNA isolation / purification

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RNA isolation / purification Bacteria - Gram positive Bacillus anthracis Experiment

The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads can be a best alternative.

RNA RNA isolation / purification Bacteria Gram positive Bacillus anthracis

RNA isolation / purification Bacteria - Gram positive Clostridium difficile Experiment

RNA RNA isolation / purification Bacteria Gram positive Clostridium difficile

RNA isolation / purification Bacteria - Gram positive Clostridium tetani Experiment

RNA RNA isolation / purification Bacteria Gram positive Clostridium tetani

RNA isolation / purification Bacteria - Gram positive Clostridum botulinum Experiment

RNA RNA isolation / purification Bacteria Gram positive Clostridum botulinum

RNA isolation / purification Bacteria - Gram positive Lactobacillus amylovorus Experiment

RNA RNA isolation / purification Bacteria Gram positive Lactobacillus amylovorus

RNA isolation / purification Bacteria - Gram positive Listeria monocytogens Experiment

RNA RNA isolation / purification Bacteria Gram positive Listeria monocytogens

RNA isolation / purification Bacteria - Gram positive Staphylococcus epidermidis Experiment

RNA RNA isolation / purification Bacteria Gram positive Staphylococcus epidermidis

RNA isolation / purification Bacteria - Gram positive Staphylococcus saprophycitius Experiment

RNA RNA isolation / purification Bacteria Gram positive Staphylococcus saprophycitius

RNA isolation / purification Bacteria - Gram positive Streptococcus pyogenes Experiment

RNA RNA isolation / purification Bacteria Gram positive Streptococcus pyogenes

RNA isolation / purification Cells - primary bovine coronary artery endothelial cells Experiment

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells primary bovine coronary artery endothelial cells

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