siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells

Get tips on using Gibco™Advanced DMEM/F-12 to perform 3D Cell Culture Media Human small intestinal organoids

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - human HMEC

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - human HUVEC

Get tips on using EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit to perform ChIP Human - Glioblastoma cell line

Get tips on using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003 to perform ChIP Human - Glioblastoma cell line

Get tips on using EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit to perform ChIP Human - Fibroblast cell lines

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

Get tips on using Imprint® Methylated DNA Quantification Kit to perform DNA methylation profiling Gene specific profiling - Mouse muscle stem cells SPRY1

Get tips on using Mesenchymal Stem Cell Growth Medium to perform Stem cell culture media Human bone mesenchymal stem cell (BMSC)