DNA isolation / purification Cells

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using QIAprep Spin Miniprep Kit to perform Plasmid Isolation Salmonella enterica serovar Indiana (S. Indiana)

Get tips on using NucleoBond® Xtra Midi / Maxi to perform Plasmid Isolation Shiga toxin-producing E. coli

Get tips on using QIAGEN Plasmid Plus 96 Miniprep Kit (4) to perform Plasmid Isolation E. coli DH5α

Get tips on using B-PER™ Bacterial Protein Extraction Reagent to perform Protein isolation Bacteria - Listeria monocytogenes

Get tips on using B-PER™ Bacterial Protein Extraction Reagent to perform Protein isolation Bacteria - Streptococcus mutans

Get tips on using B-PER™ Bacterial Protein Extraction Reagent to perform Protein isolation Bacteria - Lactobacillus casei

Get tips on using Y-PER™ Yeast Protein Extraction Reagent to perform Protein isolation Yeast - Candida tenuis

Get tips on using Y-PER™ Yeast Protein Extraction Reagent to perform Protein isolation Yeast - Spathaspora passalidarum

Get tips on using Y-PER™ Yeast Protein Extraction Reagent to perform Protein isolation Yeast - Yarrowia lipolytica